Different events involved in the induction of macrophage tumor necrosis factor by Candida albicans and lipopolysaccharide

Cell Immunol. 1994 Sep;157(2):501-9. doi: 10.1006/cimm.1994.1245.

Abstract

Using an in vitro experimental model, we have recently demonstrated that Candida albicans in its hyphal form (H-Candida), similarly to lipopolysaccharide (LPS), enhances tumor necrosis factor (TNF) secretory response in the cloned macrophage (M phi) population ANA-1. Here we show that H-Candida and LPS each differ in their requirements for intact protein kinase functions, susceptibility to 0.4-microns micropore-size membranes, and sensitivity to polymyxin B. These results, together with the synergistic effect occurring between H-Candida and LPS in inducing TNF response, indicate the existence of different receptor(s) and/or signal-transduction pathway(s) through which the two stimuli act.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Fungal / pharmacology
  • Candida albicans / immunology*
  • Candidiasis / immunology*
  • Cell Line
  • Gene Expression / drug effects
  • In Vitro Techniques
  • Lipopolysaccharides / pharmacology*
  • Macrophages / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Polymyxin B / pharmacology
  • RNA, Messenger / genetics
  • Signal Transduction
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • Antigens, Fungal
  • Lipopolysaccharides
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Polymyxin B