Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes

EMBO J. 1994 Aug 15;13(16):3801-11.

Abstract

The sequence requirements for in vivo telomere function in the fission yeast, Schizosaccharomyces pombe, have been investigated. A 258 bp tract of previously characterized cloned fission yeast terminal repeats adjacent to 800 bp of telomere-associated sequences is sufficient to seed new telomeres onto linearized ars-containing plasmids when introduced into cells. The resulting transformants contain unrearranged, acentric, linear episomes. Cloned telomeres, with and without telomere-associated sequences adjacent to the 258 bp terminal repeats, were utilized to introduce chromosome breaks at specific sites in a non-essential minichromosome. Truncated minichromosome derivatives were recovered containing the ura4 or ade6 gene adjacent to a newly formed telomere. These telomeres exert reversible position effects on the expression of the adjacent ura4 or ade6 genes.

Publication types

  • Comparative Study

MeSH terms

  • Adenine / metabolism
  • Cell Division / drug effects
  • Chromosome Aberrations / genetics*
  • DNA, Recombinant / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Extrachromosomal Inheritance
  • Gene Expression Regulation, Fungal*
  • Gene Rearrangement
  • Genes, Fungal / genetics*
  • Leucine / metabolism
  • Orotic Acid / analogs & derivatives
  • Orotic Acid / pharmacology
  • RNA, Fungal / analysis
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / growth & development
  • Uracil / metabolism

Substances

  • DNA, Recombinant
  • RNA, Fungal
  • Uracil
  • Orotic Acid
  • 5-fluoroorotic acid
  • Leucine
  • Adenine