Association of N-ethylmaleimide-sensitive factor with synaptic vesicles

FEBS Lett. 1994 Aug 22;350(2-3):253-7. doi: 10.1016/0014-5793(94)00778-0.


N-Ethylmaleimide-sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi-pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti-NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca2+ influx.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Carrier Proteins / metabolism*
  • Gene Expression
  • N-Ethylmaleimide-Sensitive Proteins
  • Protein Binding
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Synaptic Vesicles / metabolism*
  • Synaptophysin / metabolism
  • Vesicular Transport Proteins*


  • Carrier Proteins
  • RNA, Messenger
  • Synaptophysin
  • Vesicular Transport Proteins
  • N-Ethylmaleimide-Sensitive Proteins
  • Nsf protein, rat