Inhibition of rhodopsin phosphorylation by non-myristoylated recombinant recoverin

Biochem Biophys Res Commun. 1994 Aug 30;203(1):121-7. doi: 10.1006/bbrc.1994.2157.

Abstract

Bovine recoverin regulates rhodopsin phosphorylation and controls photoreceptor light sensitivity in a Ca(2+)-dependent manner. Recoverin is post-translationally modified with lipids (myristic acid or related lipids) at its N-terminus. Since with this lipid modification (N-myristoylation), recoverin associates with rod outer segment membranes in a Ca(2+)-dependent manner, N-myristoylation has been suggested to be important for the function of this protein. To study the role of this modification, we obtained recombinant non-myristoylated recoverin in E. coli and studied its functional properties. Here, we report that recombinant non-myristoylated recoverin inhibits rhodopsin phosphorylation at Ca2+ concentrations of 30 nM-10 microM in a similar way as native N-myristoylated recoverin does. Thus, our result showed that N-myristoylation is not essential for the Ca(2+)-dependent inhibition of rhodopsin phosphorylation by recoverin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Calcium / pharmacology
  • Calcium-Binding Proteins / biosynthesis
  • Calcium-Binding Proteins / isolation & purification
  • Calcium-Binding Proteins / pharmacology*
  • Cattle
  • Cell Membrane / metabolism
  • Cloning, Molecular
  • Eye Proteins*
  • Hippocalcin
  • Lipoproteins*
  • Myristic Acid
  • Myristic Acids / metabolism*
  • Nerve Tissue Proteins*
  • Phosphoproteins / antagonists & inhibitors
  • Phosphoproteins / isolation & purification
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Ranidae
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / pharmacology
  • Recoverin
  • Rhodopsin / metabolism*
  • Rod Cell Outer Segment / metabolism*

Substances

  • Calcium-Binding Proteins
  • Eye Proteins
  • Lipoproteins
  • Myristic Acids
  • Nerve Tissue Proteins
  • Phosphoproteins
  • Recombinant Proteins
  • Myristic Acid
  • Recoverin
  • Hippocalcin
  • Adenosine Triphosphate
  • Rhodopsin
  • Calcium