Tumour necrosis factor alpha and interleukin-1 beta induce specific subunits of NFKB to bind the HIV-1 enhancer: characterisation of transcription factors controlling human immunodeficiency virus type 1 gene expression in neural cells

Biochem Biophys Res Commun. 1994 Aug 30;203(1):623-30. doi: 10.1006/bbrc.1994.2228.


In human astrocytoma and neuroblastoma cell lines tumour necrosis factor alpha (TNF alpha) and interleukin 1 beta (IL-1 beta) induced NFKB and an additional KB-specific protein of approximately 80 K to bind the HIV-1 enhancer. One nucleoprotein complex contained prototypical NFKB comprising of p50 and p65 subunits and a second contained the p65 subunit and an 80 K factor, p80. Over a 24 hr period of cytokine stimulation the p50/p65 complex of NFKB was present in the nucleus whilst the second complex declined in abundance after two hours due to the decline of p80. In unstimulated cells, DNAse I footprinting revealed a previously unidentified octamer-like binding site in the negative regulatory element (NRE) of the HIV-1 long terminal repeat (LTR) which specifically bound protein factors present in human astrocytoma, neuroblastoma and murine oligodendroglioma cell lines. A second unique motif, also in the NRE, was observed with extracts from one human neuroblastoma cell line and a murine oligodendroglioma. Footprinting analysis also confirmed that Sp1, TATA, Site A and Site B motifs of the LTR were occupied by nuclear factors present in neural cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytoma
  • Base Sequence
  • Binding Sites
  • Cell Line
  • Cell Nucleus / metabolism
  • DNA, Neoplasm / metabolism
  • Deoxyribonuclease I
  • Enhancer Elements, Genetic*
  • HIV-1 / genetics*
  • HIV-1 / metabolism
  • Humans
  • Interleukin-1 / pharmacology*
  • Macromolecular Substances
  • Molecular Sequence Data
  • NF-kappa B / metabolism*
  • Neuroblastoma
  • Neurons / metabolism
  • Oligodeoxyribonucleotides
  • Transcription Factors / metabolism*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology*


  • DNA, Neoplasm
  • Interleukin-1
  • Macromolecular Substances
  • NF-kappa B
  • Oligodeoxyribonucleotides
  • Transcription Factors
  • Tumor Necrosis Factor-alpha
  • Deoxyribonuclease I