Synthesis in vitro of phi29-specific early proteins directed by phage DNA

Eur J Biochem. 1975 Feb 21;51(2):587-91. doi: 10.1111/j.1432-1033.1975.tb03960.x.


The RNA and proteins synthesized in an Escherichia coli cell-free system of protein synthesis directed by Bacillus subtilis phage phi29 DNA were studied. Hybridization-competition experiments showed that most of the RNA species synthesized in vitro are early RNAs. Many of the early proteins induced after phage infection were also synthesized in the E. coli cell-free system. None of the late proteins, structural or non-structural, were synthesized in the system in vitro.

MeSH terms

  • Bacillus subtilis / drug effects
  • Bacillus subtilis / metabolism*
  • Bacteriophages / drug effects
  • Bacteriophages / metabolism*
  • Cell-Free System
  • Chloramphenicol / pharmacology
  • DNA, Viral / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Muramidase / biosynthesis
  • Nucleic Acid Hybridization
  • Protein Biosynthesis* / drug effects
  • Time Factors
  • Transcription, Genetic* / drug effects
  • Uracil Nucleotides / metabolism
  • Viral Proteins / biosynthesis*


  • DNA, Viral
  • Uracil Nucleotides
  • Viral Proteins
  • Chloramphenicol
  • Muramidase