Cytokinin regulation of a soybean pollen allergen gene

Plant Mol Biol. 1994 Aug;25(5):829-35. doi: 10.1007/BF00028877.

Abstract

Cytokinin treatment of suspension-cultured soybean cells stimulated the accumulation of an mRNA, called cim 1, by a factor of ca. 20 within 4 h. Induction of cim 1 mRNA accumulation occurred at benzyladenine concentrations as low as 10(-8) M. Furthermore, cim 1 mRNA accumulation was stimulated in the absence of cytokinin by staurosporine (an inhibitor of protein kinases) and inhibited in the presence of cytokinin by okadaic acid (an inhibitor of protein phosphatases 1 and 2a), suggesting that cim 1 accumulation in response to cytokinin is dependent on cytokinin-induced dephosphorylation of one or more cellular proteins. The deduced amino acid sequence of the cim 1 protein product, derived from the complete nucleotide sequence of a cim 1 cDNA, was 40% identical to that of a perennial rye grass pollen allergen cDNA (Lol Pl). This sequence also indicated that the cim 1 protein product contains a putative signal peptide followed by predominantly hydrophilic residues, consistent with the hypothesis that it is exported to the apoplast.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / pharmacology
  • Allergens / biosynthesis
  • Allergens / genetics*
  • Amino Acid Sequence
  • Antigens, Plant
  • Base Sequence
  • Cells, Cultured
  • Cytokinins / pharmacology
  • Ethers, Cyclic / pharmacology
  • Gene Expression Regulation / drug effects
  • Genes, Plant / genetics*
  • Molecular Sequence Data
  • Okadaic Acid
  • Phosphoprotein Phosphatases / antagonists & inhibitors
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Pollen / genetics*
  • Pollen / immunology
  • Protein Kinase C / antagonists & inhibitors
  • RNA, Messenger / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Soybean Proteins*
  • Soybeans / cytology
  • Soybeans / genetics*
  • Soybeans / immunology
  • Staurosporine

Substances

  • Alkaloids
  • Allergens
  • Antigens, Plant
  • Cytokinins
  • Ethers, Cyclic
  • Lol p I protein, Lolium perenne
  • Plant Proteins
  • RNA, Messenger
  • Soybean Proteins
  • cim 1 protein, Glycine max
  • Okadaic Acid
  • Protein Kinase C
  • Phosphoprotein Phosphatases
  • Staurosporine

Associated data

  • GENBANK/U03860