Determinants of differential migration of mouse spleen cells treated with concanavalin A

J Immunol. 1975 Sep;115(3):771-6.


The effects of Con A on homing patterns of mouse spleen cells were investigated. After 1 to 18 hr incubation with or without the mitogen, cells were labeled with 51Cr and injected i.v. into syngeneic hosts. Treatment of spleen cells with Con A decreased localization in lymph nodes 75 to 95% and inhibited homing to recipient spleens 17 to 50%. Conversely, localization in liver and lungs was increased. Migration of cells labeled with both 125I-Con A and 51Cr revealed that cells binding the largest amount of Con A were trapped in lungs whereas cells migrating to lymph nodes and spleen had a much lower Con A content. Elution of Con A from lymphocyte surfaces with methyl-alpha-D-mannoside (MAM) reduced the percentage of cells localizing in lungs, with a concomitant increase in the percentage of cells migrating to lymph nodes. However, even after MAM treatment, a cell population with a relatively large Con A content localized in the lungs. These data indicate that both surface-bound Con A and Con A-induced changes in lymphocyte membranes inhibit migration of Con A-activated cells to recipient lymph nodes. They suggest that after Con A treatment lymph node-seeking cells do not migrate normally because of preferential sequestration in lungs of lymphocytes with either Con A-binding sites of increased density or avidity or with an increased propensity for Con A internalization.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Binding Sites
  • Cell Membrane / immunology
  • Cell Movement
  • Cell Survival
  • Chromium Radioisotopes
  • Concanavalin A*
  • Immune Sera
  • Iodine Radioisotopes
  • Liver / cytology
  • Lung / cytology
  • Lymph Nodes / cytology
  • Lymphocytes / immunology*
  • Male
  • Methylmannosides
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Rabbits / immunology
  • Spleen / cytology
  • Whole-Body Counting


  • Chromium Radioisotopes
  • Immune Sera
  • Iodine Radioisotopes
  • Methylmannosides
  • Concanavalin A