The distribution of novel intermediate filament proteins defines subpopulations of myenteric neurons in rat intestine

Gastroenterology. 1994 Sep;107(3):666-74. doi: 10.1016/0016-5085(94)90113-9.

Abstract

Background/aims: Recent studies with neurofilament antibodies as neuronal markers have shown subpopulations of myenteric neurons that do not contain neurofilament proteins. Novel neuronal intermediate filament proteins alpha-internexin, peripherin, and nestin have been identified. The aim of this study was to examine the distribution of these novel intermediate filaments in comparison with neurofilaments in myenteric plexus neurons.

Methods: Using indirect immunofluorescence techniques in whole-mount cryostat sections from neonate and adult rat small intestine and in primary cultures of myenteric neurons, the distribution of neurofilaments, alpha-internexin, peripherin, and nestin was studied in comparison with the neuronal marker protein gene product (PGP) 9.5 in myenteric neurons.

Results: Sixty-five percent of neurons contained neurofilament triplet proteins. alpha-Internexin and/or peripherin were found in the neurofilament-negative neurons. PGP 9.5 was present in 80% of the myenteric neurons. Of the neurons that were PGP negative, > 95% contained peripherin or alpha-internexin. Nestin was not found in either neonate or adult myenteric neurons but was seen in glial cells in culture.

Conclusions: The results suggest that a subpopulation of myenteric neurons lacks neurofilament triplet proteins but contains either peripherin, alpha-internexin, or both. This selective distribution of intermediate filaments in subpopulations of enteric neurons may support differential roles in these structurally unique neurons.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Fluorescent Antibody Technique
  • Intermediate Filament Proteins / metabolism*
  • Intestine, Small / innervation*
  • Intestine, Small / metabolism*
  • Myenteric Plexus / cytology
  • Myenteric Plexus / physiology*
  • Neurons / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Thiolester Hydrolases / metabolism
  • Tissue Distribution
  • Ubiquitin Thiolesterase

Substances

  • Intermediate Filament Proteins
  • Thiolester Hydrolases
  • Ubiquitin Thiolesterase