Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract

Mol Gen Genet. 1994 Aug 15;244(4):444-50. doi: 10.1007/BF00286698.


Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mutY-dependent repair tract was measured by the specific incorporation of alpha-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3' side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Glycosylases*
  • DNA Polymerase I / metabolism*
  • DNA Repair*
  • DNA, Bacterial / metabolism*
  • Escherichia coli / genetics*
  • Molecular Sequence Data
  • N-Glycosyl Hydrolases / metabolism*


  • DNA, Bacterial
  • DNA Polymerase I
  • DNA Glycosylases
  • N-Glycosyl Hydrolases
  • mutY adenine glycosylase