A monoclonal antibody to the human c-erbB3 protein stimulates the anchorage-independent growth of breast cancer cell lines

Br J Cancer. 1994 Sep;70(3):459-65. doi: 10.1038/bjc.1994.328.

Abstract

The c-erbB3 protein is a member of the type I growth factor receptor family. It has a widespread pattern of expression in normal tissues and is overexpressed in about 20% of breast cancers. We have raised a specific monoclonal antibody, called SGP1, against the extracellular domain of c-erbB3 which recognises the native form of the protein. The monoclonal antibody was found to modestly but significantly stimulate the anchorage-independent cloning efficiency of the breast tumour cell lines BT483 and T47D, both of which express the c-erbB3 protein. No effect was observed on 293 cells lacking expression, nor did a control isotype-matched antibody promote the growth of any of the cells tested. These results suggest that the c-erbB3 protein may normally act as a growth factor receptor.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology*
  • Antibody Specificity
  • Blotting, Western
  • Breast Neoplasms / pathology*
  • Cell Adhesion / drug effects
  • Cell Division / drug effects
  • ErbB Receptors / immunology*
  • ErbB Receptors / metabolism
  • ErbB Receptors / physiology*
  • Humans
  • Immunization
  • Mice
  • Mice, Inbred BALB C
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins / immunology*
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins / physiology*
  • Receptor, ErbB-3
  • Stimulation, Chemical
  • Tumor Cells, Cultured / drug effects

Substances

  • Antibodies, Monoclonal
  • Proto-Oncogene Proteins
  • ErbB Receptors
  • Protein-Tyrosine Kinases
  • Receptor, ErbB-3