Tyrosine phosphorylation of beta-catenin and plakoglobin enhanced by hepatocyte growth factor and epidermal growth factor in human carcinoma cells

Cell Adhes Commun. 1994 Jan;1(4):295-305. doi: 10.3109/15419069409097261.


The effect of hepatocyte growth factor/scatter factor (HGF/SF) and epidermal growth factor (EGF) on cadherin-mediated adhesion of human carcinoma cells was studied. HGF/SF induced scattering of colonic adenocarcinoma HT29 and gastric adenocarcinomas MKN7 and MKN74 cells. Likewise, EGF induced scattering of HT29 and MKN7 cells. These cells expressed E-cadherin, which was concentrated at cell-cell contact sites. When the scattering of these cells was induced by HGF/SF or EGF, the E-cadherin concentration at cell-cell boundaries tended to decrease. Immunoblotting analyses, however, demonstrated that these growth factor treatments did not alter the expression of E-cadherin and E-cadherin-associated proteins, alpha- and beta-catenin and plakoglobin. beta-Catenin, plakoglobin and an unidentified 115-kDa molecule associated with E-cadherin were found to be phosphorylated at tyrosine residues, and these phosphorylations were enhanced by the growth factor treatments. These results suggest that HGF/SF and EGF may modulate the function of the cadherin-catenin system via tyrosine phosphorylation of cadherin-associated proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadherins / metabolism
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cytoskeletal Proteins / metabolism*
  • Desmoplakins
  • Epidermal Growth Factor / pharmacology*
  • Hepatocyte Growth Factor / pharmacology*
  • Humans
  • Neoplasm Invasiveness / physiopathology
  • Phosphorylation
  • Trans-Activators*
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / metabolism
  • Tyrosine / metabolism
  • alpha Catenin
  • beta Catenin
  • gamma Catenin


  • CTNNA1 protein, human
  • CTNNB1 protein, human
  • Cadherins
  • Cytoskeletal Proteins
  • Desmoplakins
  • Trans-Activators
  • alpha Catenin
  • beta Catenin
  • gamma Catenin
  • Tyrosine
  • Epidermal Growth Factor
  • Hepatocyte Growth Factor