Interleukin 2-induced activation of JAK3: possible involvement in signal transduction for c-myc induction and cell proliferation

FEBS Lett. 1994 Sep 5;351(2):201-6. doi: 10.1016/0014-5793(94)00831-0.


We have investigated the role of JAK3 in interleukin 2 (IL-2)-induced signal transduction with a human T cell line, ED40515(-), lacking expression of the IL-2 receptor gamma chain and its sublines transfected with wild-type or mutant cDNAs of the IL-2 receptor gamma chain. Our results demonstrated that the membrane-proximal cytoplasmic region, encompassing the src homology region 2 (SH2)-like subdomain, of the gamma chain is essential for association and activation of JAK3. Furthermore, IL-2-induced activation of JAK3 paralleled induction of the c-myc gene and DNA synthesis but not induction of the c-fos and c-jun genes. These results support the hypothesis that JAK3 plays a pivotal role in the IL-2 receptor-mediated signals for cell growth.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Cell Line, Transformed
  • Enzyme Activation
  • Gene Expression Regulation
  • Genes, myc*
  • Humans
  • Interleukin-2 / pharmacology*
  • Janus Kinase 2
  • Janus Kinase 3
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism*
  • Proto-Oncogene Proteins c-myc / biosynthesis
  • Proto-Oncogene Proteins*
  • Receptors, Interleukin-2 / genetics
  • Signal Transduction*
  • T-Lymphocytes / drug effects*


  • Interleukin-2
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myc
  • Receptors, Interleukin-2
  • Protein-Tyrosine Kinases
  • JAK2 protein, human
  • JAK3 protein, human
  • Janus Kinase 2
  • Janus Kinase 3