SNAP-25 is present in a SNARE complex in adrenal chromaffin cells

FEBS Lett. 1994 Sep 5;351(2):207-10. doi: 10.1016/0014-5793(94)00833-7.

Abstract

SNAP-25 (synaptosomal-associated protein 25 kDa) is a target for botulinum neurotoxins A and E, which both inhibit neurotransmitter release, and was recently identified together with syntaxin and synaptobrevin as receptors for NSF and alpha-SNAP. We show that SNAP-25 was enriched in the microsomal fraction from adrenal medulla, although the level of SNAP-25 in adrenal medullary microsomes was about 20-fold less than in brain microsomes. Immunocytochemistry confirmed the presence of SNAP-25 in cultured chromaffin cells and showed plasma membrane staining. Using immunoprecipitation, we found that SNAP-25 was present in a complex with syntaxin, synaptobrevin, synaptotagmin, NSF, alpha-SNAP and other unidentified polypeptides. These data indicate that SNAP-25 in chromaffin cells is present in a complex similar to that identified in brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Medulla / chemistry*
  • Amino Acid Sequence
  • Animals
  • Calcium-Binding Proteins*
  • Carrier Proteins / analysis
  • Cattle
  • Cell Membrane / chemistry
  • Fluorescent Antibody Technique
  • Membrane Glycoproteins / analysis
  • Membrane Proteins / analysis
  • Membrane Proteins / isolation & purification*
  • Microsomes / chemistry
  • Molecular Sequence Data
  • N-Ethylmaleimide-Sensitive Proteins
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / isolation & purification*
  • Precipitin Tests
  • R-SNARE Proteins
  • Synaptosomal-Associated Protein 25
  • Synaptotagmins
  • Vesicular Transport Proteins*

Substances

  • Calcium-Binding Proteins
  • Carrier Proteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • R-SNARE Proteins
  • Synaptosomal-Associated Protein 25
  • Vesicular Transport Proteins
  • Synaptotagmins
  • N-Ethylmaleimide-Sensitive Proteins