Molecular cloning of a novel candidate G protein-coupled receptor from rat brain

FEBS Lett. 1994 Sep 12;351(3):375-9. doi: 10.1016/0014-5793(94)00888-4.


A PCR cloning strategy using primers designed from sequences selectively conserved among a cannabinoid receptor and two orphan receptors, was used to isolate novel G protein-coupled receptors. rCNL3, a 1.75 kb cDNA encoding a 363 amino acid protein, was isolated from a rat cerebral cortex library. Sequence analysis showed that rCNL3 possesses a number of structural characteristics of G protein-coupled receptors and has 61% amino acid identity (from transmembrane region one through the carboxyl-terminus) with two other candidate G protein-coupled receptors. Therefore, these three receptors may comprise a receptor subfamily with identical or closely related endogenous ligands. Northern and in situ hybridization experiments demonstrated that rCNL3 mRNA is expressed in the rat brain, with a prominent distribution in striatum.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Corpus Striatum / metabolism*
  • DNA, Complementary
  • GTP-Binding Proteins / metabolism*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Cannabinoid
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, Drug / genetics
  • Receptors, Drug / metabolism
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Cannabinoid
  • Receptors, Cell Surface
  • Receptors, Drug
  • GTP-Binding Proteins

Associated data

  • GENBANK/U12006