Individualisation of two new genomic groups among American Borrelia burgdorferi sensu lato strains

FEMS Microbiol Lett. 1994 Aug 1;121(1):93-8. doi: 10.1111/j.1574-6968.1994.tb07081.x.

Abstract

We developed a quick typing method for Borrelia burgdorferi sensu lato strains using a fla gene-based PCR assay, followed by dot blot hybridization with non-radioactive species-specific probes. Thirty-six out of 46 strains belonged to one of the four described species (B. burgdorferi sensu stricto n = 11, B. garinii n = 11, B. afzelii n = 9 and B. japonica n = 5) and hybridized with its own species-specific probe. Among the 10 remaining American strains, two new additional genomic groups were identified. This finding was confirmed by direct sequencing of the fla gene-derived amplicons and whole DNA hybridization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Borrelia burgdorferi Group / classification*
  • Borrelia burgdorferi Group / genetics*
  • Flagellin / genetics
  • Genes, Bacterial / genetics
  • Genome, Bacterial
  • Humans
  • Lyme Disease / epidemiology
  • Lyme Disease / microbiology
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Siphonaptera / microbiology
  • Ticks / microbiology
  • United States / epidemiology

Substances

  • Flagellin

Associated data

  • GENBANK/L29200
  • GENBANK/L29234
  • GENBANK/L29235
  • GENBANK/L29236
  • GENBANK/L29237
  • GENBANK/L29238
  • GENBANK/L29239
  • GENBANK/L29240
  • GENBANK/L29241
  • GENBANK/L29242
  • GENBANK/L29243
  • GENBANK/L29244
  • GENBANK/L29245