Determination of arginase activity in macrophages: a micromethod

J Immunol Methods. 1994 Sep 14;174(1-2):231-5. doi: 10.1016/0022-1759(94)90027-2.

Abstract

We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25,000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mumol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginase / analysis*
  • Bone Marrow Cells
  • Female
  • Macrophage Activation
  • Macrophages / enzymology*
  • Mice
  • Microchemistry
  • Urea / analysis

Substances

  • Urea
  • Arginase