Tropism for primary monocytes and for monocytoid cell lines are separate features of HIV-1 variants

J Leukoc Biol. 1994 Sep;56(3):225-9. doi: 10.1002/jlb.56.3.225.


We have analyzed the phenotype of five human monocytoid cell lines (U-937, THP-1, RC.2A, Mono Mac 6, and DD) and their capacity to support replication of HIV-1 strains with known tropism for T lymphocytes and mononuclear phagocytes. HIV-1 Ba-L, a prototype monocytotropic HIV-1 strain, was unable to establish continuous replication in any of the cell lines used. In contrast, the lymphotropic strain IIIB could replicate in all CD4-positive cell lines. We conclude that the capacity of HIV-1 isolates to replicate in established human monocytoid malignant cells does not correlate with the tropism of the virus for primary mononuclear phagocytes. Since monocytoid cell lines do not distinguish HIV-1 variants tropic for mononuclear phagocytes, we suggest the use of primary cells for studies of HIV-1 tropism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acquired Immunodeficiency Syndrome / pathology
  • Acquired Immunodeficiency Syndrome / physiopathology
  • Cell Line
  • Cell Movement / physiology
  • Cytokines / metabolism
  • Fluorescent Antibody Technique
  • HIV-1 / isolation & purification
  • HIV-1 / physiology*
  • Humans
  • In Vitro Techniques
  • Leukemia, Monocytic, Acute / pathology
  • Leukemia, Myelomonocytic, Acute / pathology
  • Lymphocytes / cytology
  • Lymphocytes / physiology
  • Lymphoma, Large B-Cell, Diffuse / pathology
  • Monocytes / cytology
  • Monocytes / microbiology
  • Monocytes / physiology*
  • Phagocytes / cytology
  • Phagocytes / microbiology
  • Phagocytes / physiology
  • Phenotype
  • T-Lymphocytes / cytology
  • T-Lymphocytes / microbiology
  • T-Lymphocytes / physiology
  • Tumor Cells, Cultured
  • Virus Replication


  • Cytokines