A novel glucose-6-phosphate dehydrogenase in Plasmodium falciparum: cDNA and primary protein structure

Biochim Biophys Acta. 1994 Sep 13;1219(1):191-4. doi: 10.1016/0167-4781(94)90269-0.

Abstract

The structure of the parasite-encoded G6PD (PfG6PD) may provide clues about the relative protection against malaria in humans with glucose-6-phosphate dehydrogenase (G6PD) deficiency. We have cloned Pfg6pd cDNA encoding a predicted 856 amino acid residues polypeptide with a calculated molecular mass of > 94 kDa. The predicted amino acid sequence is highly homologous to G6PD from other organisms. Pfg6pd maps as a single or low copy number gene to chromosome 14. The unusually large N-terminus and the distance between the NADP-binding site and G6PD-binding site is novel for the parasite G6PD. The differences between parasite and human G6PD proteins could potentially be exploited for designing new chemotherapeutic agents.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Glucose-6-Phosphate
  • Glucosephosphate Dehydrogenase / genetics*
  • Glucosephosphates / metabolism
  • Molecular Sequence Data
  • NADP / metabolism
  • Plasmodium falciparum / enzymology
  • Plasmodium falciparum / genetics*
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Glucosephosphates
  • NADP
  • Glucose-6-Phosphate
  • Glucosephosphate Dehydrogenase

Associated data

  • GENBANK/M80655