Temporal Order of S Phase and Mitosis in Fission Yeast Is Determined by the State of the p34cdc2-mitotic B Cyclin Complex

Cell. 1994 Sep 9;78(5):813-22. doi: 10.1016/s0092-8674(94)90542-8.

Abstract

We show here that the state of the p34cdc2-p56cdc13 mitotic B cyclin complex determines whether a fission yeast cell undergoes S phase or mitosis. Mutants defective for p56cdc13 reset to G1 and rereplicate their DNA, while cells completely lacking the p34cdc2-p56cdc13 complex undergo multiple rounds of S phase. In contrast, formation of the p34cdc2-p56cdc13 complex in G1 promotes cells inappropriately into mitosis. We propose that the temporal order of S phase and mitosis is maintained by the presence or absence of the p34cdc2-p56cdc13 complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CDC2 Protein Kinase / genetics
  • CDC2 Protein Kinase / metabolism*
  • Cell Compartmentation
  • Cyclin B
  • Cyclins / genetics
  • Cyclins / metabolism*
  • DNA Replication / physiology
  • G1 Phase / physiology
  • Mitosis / genetics
  • Mitosis / physiology*
  • Models, Biological
  • Mutation
  • Protein Binding
  • Recombinant Proteins / metabolism
  • S Phase / genetics
  • S Phase / physiology*
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / growth & development*
  • Time Factors

Substances

  • Cyclin B
  • Cyclins
  • Recombinant Proteins
  • CDC2 Protein Kinase