Targeting of an enhancer trap to vestigial

Dev Biol. 1994 Sep;165(1):290-3. doi: 10.1006/dbio.1994.1254.

Abstract

The vestigial gene (vg+) is required for normal wing development and is expressed in a spatially distinct pattern in imaginal discs. We have exploited a general property of P element alleles to target an enhancer trap to the 5' region of the gene. By replacing the P element resident at this site in vg21 with a P element carrying a lacZ reporter gene, the vglacZ1 allele was selected on the basis of its increased mutant phenotype. In contrast to vg+ expression, which occurs primarily in the presumptive wing margin and hinge, beta-galactosidase expression in vglacZ1 wing discs is localized to the dorsal wing surface and displays homologous haltere expression. The targeting of P element enhancer traps could be readily extended to other genes with low rates of primary P element insertion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • DNA
  • DNA Transposable Elements
  • Drosophila / embryology
  • Drosophila / genetics*
  • Enhancer Elements, Genetic*
  • Larva
  • Molecular Sequence Data
  • Polymerase Chain Reaction

Substances

  • DNA Transposable Elements
  • DNA