Effects of metadoxine on cellular free fatty acid levels in ethanol treated rats

Int J Tissue React. 1993;15(6):235-43.


Free fatty acids (FFA) and fatty-acid ethyl esters (FAEE) were extracted from different organs of rat after ethanol administration. The results showed that ethanol treatment increased the concentration of saturated and mono-unsaturated FFA and FAEE while decreasing the poly-unsaturated fatty-acid content. Pretreatment of animals with Metadoxine one hour before ethanol administration inhibited the increased formation of saturated and mono-unsaturated FFA. While ethanol administration induced a marked increase in glutathione (GSH) S-transferase activity correlated with increased formation of FAEE in different rat tissues examined, pretreatment of such rats with Metadoxine reduced considerably the increased enzyme activity of their GSH S-transferase. Elevated concentrations of saturated and mono-unsaturated FFA conceivably reflect changes in the composition of lipid membranes. Hence, the cellular response to ethanol would be the formation of highly-packed rigid membrane structures. Pretreatment with Metadoxine causes an inhibition in the formation of elevated quantities of saturated and mono-unsaturated fatty acids. This condition could result in a lower degree of adaptability to ethanol intake and perhaps a decreased level of tolerance to ethanol.

MeSH terms

  • Animals
  • Cells / drug effects
  • Cells / metabolism
  • Drug Combinations
  • Drug Evaluation, Preclinical
  • Ethanol / pharmacology*
  • Fatty Acids / metabolism*
  • Fatty Acids, Nonesterified / metabolism*
  • Male
  • Pyridoxine / pharmacology*
  • Pyrrolidonecarboxylic Acid / pharmacology*
  • Random Allocation
  • Rats
  • Rats, Wistar


  • Drug Combinations
  • Fatty Acids
  • Fatty Acids, Nonesterified
  • Ethanol
  • metadoxine
  • Pyridoxine
  • Pyrrolidonecarboxylic Acid