Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 91 (19), 8955-9

Isolation of Phytoalexin-Deficient Mutants of Arabidopsis Thaliana and Characterization of Their Interactions With Bacterial Pathogens

Affiliations

Isolation of Phytoalexin-Deficient Mutants of Arabidopsis Thaliana and Characterization of Their Interactions With Bacterial Pathogens

J Glazebrook et al. Proc Natl Acad Sci U S A.

Abstract

A genetic approach was used to assess the extent to which a particular plant defense response, phytoalexin biosynthesis, contributes to Arabidopsis thaliana resistance to Pseudomonas syringae pathogens. The A. thaliana phytoalexin, camalexin, accumulated in response to infection by various P. syringae strains. No correlation between pathogen avirulence and camalexin accumulation was observed. A biochemical screen was used to isolate three mutants of A. thaliana ecotype Columbia that were phytoalexin deficient (pad mutants). The mutations pad1, pad2, and pad3 were found to be recessive alleles of three different genes. pad1 and pad2 were mapped to chromosome IV and pad3 was mapped to chromosome III. Infection of pad mutant plants with strains carrying cloned avirulence genes revealed that the pad mutations did not affect the plants' ability to restrict the growth of these strains. This result strongly suggests that in A. thaliana, phytoalexin biosynthesis is not required for resistance to avirulent P. syringae pathogens. Two of the pad mutants displayed enhanced sensitivity to isogenic virulent P. syringae pathogens, suggesting that camalexin may serve to limit the growth of virulent bacteria.

Similar articles

See all similar articles

Cited by 129 PubMed Central articles

See all "Cited by" articles

References

    1. Nature. 1993 Jan 14;361(6408):153-6 - PubMed
    1. Plant Cell. 1993 Aug;5(8):865-75 - PubMed
    1. Plant J. 1993 Aug;4(2):403-10 - PubMed
    1. Plant Cell. 1991 Jan;3(1):61-72 - PubMed
    1. Cell. 1989 Jan 27;56(2):215-24 - PubMed

Publication types

LinkOut - more resources

Feedback