Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models

Eur J Biochem. 1975 Sep 15;57(2):353-9. doi: 10.1111/j.1432-1033.1975.tb02308.x.


The interaction between the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61 and beta-lactam antibiotics is a multistep process during which a rather stable enzyme - antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver-Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver-Burk plots can be obtained on the basis of a non-competitive type of inhibition. At present, a choice between the two models cannot be made.

MeSH terms

  • Acyltransferases / metabolism*
  • Binding Sites
  • Binding, Competitive
  • Carbenicillin / metabolism
  • Carboxypeptidases / metabolism*
  • Kinetics
  • Mathematics
  • Models, Chemical
  • Penicillin G / metabolism
  • Penicillin V / metabolism
  • Penicillins / metabolism*
  • Peptidyl Transferases / metabolism*
  • Protein Binding
  • Streptomyces / enzymology*


  • Penicillins
  • Acyltransferases
  • Peptidyl Transferases
  • Carboxypeptidases
  • Carbenicillin
  • Penicillin G
  • Penicillin V