Cisplatin generates superoxide anion by interaction with DNA in a cell-free system

Biochem Biophys Res Commun. 1994 Sep 15;203(2):1175-80. doi: 10.1006/bbrc.1994.2306.


Cisplatin [cis-Diamminedichloroplatinum (II), DDP] generated superoxide anion (O2-), as measured by chemiluminescence from a Cypridina luciferin analog (CLA), by interaction with calf thymus DNA (ct-DNA) in phosphate-buffered saline (PBS) at pH 7.6. The intensity of chemiluminescence of CLA (CCLA) increased with increased concentration of DDP, ct-DNA or CLA in the limited range of concentrations of these agents tested. DDP-induced CCLA was completely inhibited by the addition of an O2- scavenger, superoxide dismutase (SOD), or an antioxidant, ascorbic acid. Heat-inactivated SOD did not affect CCLA. The hydroxyl radical (OH.) scavengers, mannitol, ethanol, and formic acid, also caused a partial suppression of CCLA, suggesting that DDP produced OH. as well as O2- in the cell-free system used.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anions
  • Cattle
  • Cell-Free System
  • Cisplatin / pharmacology*
  • DNA / drug effects
  • DNA / metabolism*
  • Free Radical Scavengers
  • Hot Temperature
  • Hydroxyl Radical / metabolism
  • Luminescent Measurements
  • Pyrazines / metabolism
  • Pyrazines / pharmacology
  • Superoxide Dismutase / pharmacology
  • Superoxides / metabolism*


  • Anions
  • Free Radical Scavengers
  • Pyrazines
  • 2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one
  • Superoxides
  • Hydroxyl Radical
  • DNA
  • Superoxide Dismutase
  • Cisplatin