Co-amplification of c-myc/pvt-1 in immortalized mouse B-lymphocytic cell lines results in a novel pvt-1/AJ-1 transcript

Int J Cancer. 1993 Feb 1;53(3):493-8. doi: 10.1002/ijc.2910530323.


In a series of mouse pvt-1 cDNA clones prepared from an immortalized B-cell line that contains an amplified c-myc/pvt-1 region, we have identified a unique cDNA, AJ-I, which contains 57 bp of pvt-1 sequence (pvt-1a) spliced to a novel sequence of 2.1 kb. We report here that this 3' segment (termed AJ-IX) maps more than 60 kb telomeric to pvt-1a and is encoded by a novel locus (AJ-I) on mouse chromosome 15. The AJ-IX probe detects a transcript that is expressed only in normal mouse brain and testis. Several mast-cell tumors, Ly-I+ B-lymphocytic cell lines and a neuroblastoma also display abundant levels of AJ-IX-specific mRNA. However, splicing of pvt-1a to AJ-IX is found exclusively in Ly-I+ B-cell lines that contain amplified c-myc/pvt-1. We conclude that some features in the generation of this amplicon facilitates the synthesis of a pvt-1/AJ-IX chimeric mRNA that may play a role in immortalization of these Ly-I+ B-cell lines.

MeSH terms

  • Animals
  • B-Lymphocytes / physiology*
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • DNA / genetics
  • DNA Probes / genetics*
  • Gene Amplification
  • Gene Expression
  • Genes, myc*
  • Mice
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Messenger / genetics


  • DNA Probes
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA