Human peripheral gamma delta T cells recognize hsp60 molecules on Daudi Burkitt's lymphoma cells

J Immunol. 1993 Mar 1;150(5):2046-55.


Studies with the use of polyclonal rabbit antiserum reactive with 60-kDa heat shock proteins (hsp60) suggested that hsp60-related molecules could be found on the surface of Daudi cells and were involved in their recognition by certain human gamma delta T cells. The present study confirms this finding by using a mAb specifically recognizing hsp60. This mAb can block outgrowth of human gamma delta T cells in response to stimulation with Daudi and in response to an extract of the mycobacteria H37Ra. This anti-hsp60 mAb stains the surface of Daudi cells, but does not stain either Raji or EBV-transformed B cells, cells which do not stimulate gamma delta T cell outgrowth. Anti-hsp60 mAb could immunoprecipitate a 60-kDa molecule from the H37Ra extract but was unable to precipitate this 60-kDa molecule if the mAb was first absorbed on Daudi cells. This mAb also precipitated a 60-kDa molecule from the surface of Daudi cells which shows an electrophoretic mobility pattern consistent with hsp60. These experiments demonstrate that human gamma delta T cells recognize hsp60-related epitopes on the surface of Daudi cells and within mycobacterial extracts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Bacterial Proteins / immunology
  • Burkitt Lymphoma / immunology*
  • Chaperonin 60
  • Cross Reactions
  • Heat-Shock Proteins / immunology*
  • Humans
  • Lymphocyte Activation
  • Mice
  • Mycobacterium / immunology*
  • Rabbits
  • Receptors, Antigen, T-Cell, gamma-delta / analysis*
  • T-Lymphocytes / immunology*
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Bacterial Proteins
  • Chaperonin 60
  • Heat-Shock Proteins
  • Receptors, Antigen, T-Cell, gamma-delta