A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs

FEBS Lett. 1993 Mar 8;318(3):325-30. doi: 10.1016/0014-5793(93)80539-7.


A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat sequence, but displays one less consensus site for asparagine N-linked glycosylation and one more consensus site for phosphorylation by protein kinase C. The human SVMT gene maps to chromosome 10q25 using Southern blotting analysis of human/rodent hybrid cell lines and fluorescent in situ hybridization approaches. The cDNA, and a subclone, recognize TaqI polymorphisms that may prove useful to assess this gene's involvement in neuropsychiatric disorders involving monoaminergic brain systems.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 10*
  • Consensus Sequence
  • DNA / chemistry
  • DNA / genetics*
  • Deoxyribonucleases, Type II Site-Specific*
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • Glycosylation
  • Humans
  • In Situ Hybridization
  • Membrane Glycoproteins*
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Neuropeptides*
  • Phosphorylation
  • Polymorphism, Restriction Fragment Length*
  • Protein Kinase C / metabolism
  • Rats
  • Sequence Homology, Amino Acid
  • Synaptic Vesicles / chemistry*
  • Vesicular Biogenic Amine Transport Proteins
  • Vesicular Monoamine Transport Proteins


  • Glycoproteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Neuropeptides
  • SLC18A2 protein, human
  • Vesicular Biogenic Amine Transport Proteins
  • Vesicular Monoamine Transport Proteins
  • DNA
  • Protein Kinase C
  • Deoxyribonucleases, Type II Site-Specific
  • TCGA-specific type II deoxyribonucleases

Associated data

  • GENBANK/L09118