Bacillus Subtilis FlhA: A Flagellar Protein Related to a New Family of Signal-Transducing Receptors

Mol Microbiol. 1993 Mar;7(5):735-43. doi: 10.1111/j.1365-2958.1993.tb01164.x.

Abstract

The Bacillus subtilis flhA gene lies in the major che/fla operon, a transcription unit that spans 26 kilobases (kb) of DNA. flhA encodes a 677-amino-acid polypeptide that is a strong candidate for an integral membrane protein. The sequence of FlhA displays substantial homology to a newly identified family of putative signal-transducing receptors that have been implicated in diverse cellular processes. FlhA is the first member of this family to be described from a Gram-positive bacterium. We demonstrate that flhA is a flagellar gene and that FlhA is required in trans for the formation of products from some, but not all, B. subtilis motility-related operons that are regulated by the sigma D form of RNA polymerase. We suggest that FlhA is a component of a signalling system that is involved with the formation of some flagellar gene products during the biosynthesis of the flagellum.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / genetics
  • Bacillus subtilis / metabolism*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Cell Movement
  • Fimbriae, Bacterial / metabolism*
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Mutagenesis, Site-Directed
  • Operon
  • Recombinant Fusion Proteins / metabolism
  • Regulatory Sequences, Nucleic Acid
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction*

Substances

  • Bacterial Proteins
  • FlhA protein, Bacteria
  • Membrane Proteins
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/X63698