Restriction fragment length polymorphism analysis of Fusobacterium necrophorum using a novel repeat DNA sequence and a 16S rRNA gene probe

FEMS Microbiol Lett. 1993 Mar 1;107(2-3):205-10. doi: 10.1111/j.1574-6968.1993.tb06031.x.

Abstract

A repeated DNA sequence was isolated from Fusobacterium necrophorum biotype AB, strain FnS1. The repeated sequence shared considerable homology with the transposase gene from the Pseudomonas syringiae insertion sequence IS801. The repeat sequence was used together with a 16S ribosomal RNA gene probe to type F. necrophorum isolates using restriction fragment length polymorphisms. The probes revealed differences between several clinical isolates and will be useful tools to study the epidemiology of ovine foot abscess and other diseases caused by F. necrophorum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • DNA, Bacterial / genetics*
  • Fusobacterium / enzymology
  • Fusobacterium / genetics*
  • Molecular Sequence Data
  • Nucleotidyltransferases / genetics
  • Polymorphism, Restriction Fragment Length
  • RNA Probes
  • RNA, Ribosomal, 16S / genetics*
  • Repetitive Sequences, Nucleic Acid*
  • Sequence Homology, Amino Acid
  • Transposases

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • RNA Probes
  • RNA, Ribosomal, 16S
  • Nucleotidyltransferases
  • Transposases