Effect of serotonin on protein phosphorylation in the central nervous system of the leech Hirudo medicinalis

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Jan;104(1):125-31. doi: 10.1016/0742-8413(93)90123-3.

Abstract

1. Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology
  • Animals
  • Central Nervous System / drug effects*
  • Central Nervous System / metabolism
  • Ganglia / drug effects
  • Ganglia / metabolism
  • Leeches / drug effects*
  • Leeches / metabolism
  • Nerve Tissue Proteins / metabolism*
  • Phorbol 12,13-Dibutyrate / pharmacology
  • Phosphoproteins / analysis
  • Phosphorylation
  • Serotonin / pharmacology*

Substances

  • Nerve Tissue Proteins
  • Phosphoproteins
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Serotonin
  • Phorbol 12,13-Dibutyrate