Intercellular adhesion molecule-1 on cultured human melanoma cells: influence of cytokines

J Dermatol. 1993 Mar;20(3):144-50. doi: 10.1111/j.1346-8138.1993.tb03849.x.

Abstract

Adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and its counter-receptor, lymphocyte function associated antigen-1 (LFA-1), play very important roles in immune responses. In this study, the effects of cytokines on cultured human melanoma cells (MMG2) were examined, especially focussing on the expression of ICAM-1 on MMG2 and lymphocyte adhesion to MMG2. Both the expression of ICAM-1 and HLA-DR on MMG2 increased after treatment with IFN-gamma. ICAM-1 expression began to increase earlier than HLA-DR expression. TNF-alpha and IL-1 beta also increased the expression of ICAM-1 on MMG2. However, these cytokines did not increase the expression of HLA-DR. IFN-gamma had a dose dependent effect on lymphocyte adhesion to MMG2. Pretreatment of IFN-gamma treated MMG2 with 84H10 (anti-ICAM-1 antibody) or pretreatment of lymphocytes with either SPV-L7 (anti-LFA-1 alpha antibody) or IOT10 (anti-LFA-1 beta antibody) inhibited the lymphocyte adhesion to MMG2. These results suggest that ICAM-1 molecules induced on melanoma cells by IFN-gamma can interact with LFA-1 molecules on lymphocytes.

MeSH terms

  • Antigens, CD / analysis*
  • Antigens, CD / genetics
  • Cell Adhesion / drug effects
  • Cell Adhesion Molecules / analysis*
  • Cell Adhesion Molecules / genetics
  • Cytokines / pharmacology*
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Gene Expression / drug effects
  • HLA-DR Antigens / analysis
  • HLA-DR Antigens / genetics
  • Humans
  • Intercellular Adhesion Molecule-1
  • Interferon Type I / pharmacology
  • Interferon-beta / pharmacology
  • Interferon-gamma / pharmacology
  • Interleukin-1 / pharmacology
  • Interleukin-2 / pharmacology
  • Lymphocyte Function-Associated Antigen-1 / pharmacology
  • Lymphocytes / pathology
  • Melanoma / pathology*
  • Recombinant Proteins
  • Skin Neoplasms / pathology*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Antigens, CD
  • Cell Adhesion Molecules
  • Cytokines
  • HLA-DR Antigens
  • Interferon Type I
  • Interleukin-1
  • Interleukin-2
  • Lymphocyte Function-Associated Antigen-1
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Intercellular Adhesion Molecule-1
  • Interferon-beta
  • Interferon-gamma