A G-protein beta-subunit is essential for Dictyostelium development

Genes Dev. 1993 Jun;7(6):986-95. doi: 10.1101/gad.7.6.986.

Abstract

Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein beta-subunit and present here a more complete analysis of this gene. Low-stringency Southern blots and RFLP mapping studies suggest that the beta-subunit is a unique gene found on linkage group II. Its deduced amino acid sequence of 347 residues is approximately 60% identical to those of the human, Drosophila, and Caenorhabditis elegans beta-subunits. The carboxy-terminal 300 residues are about 70% identical; the amino-terminal 50 residues are quite divergent, containing only 10 identities. At all stages of growth and development, a single 1.9-kb beta-subunit mRNA is present at a high level, and a specific antibody detects a single 37-kD protein. We propose that G-protein heterotrimers are formed when this beta-subunit couples with each of the eight distinct G-protein alpha-subunits that are transiently expressed during development. Targeted disruption of the beta-subunit gene had no effect on the viability of haploid cells, but resulted in the inability of cells to aggregate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • DNA, Antisense / genetics
  • DNA, Fungal / analysis
  • DNA, Fungal / chemistry
  • DNA, Protozoan / analysis
  • DNA, Protozoan / chemistry
  • Dictyostelium / genetics
  • Dictyostelium / growth & development*
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / physiology*
  • Gene Expression*
  • Molecular Sequence Data
  • Mutagenesis
  • Oligonucleotides / chemistry
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Peptide Fragments / physiology*
  • Polymorphism, Restriction Fragment Length
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction

Substances

  • DNA, Antisense
  • DNA, Fungal
  • DNA, Protozoan
  • Oligonucleotides
  • Peptide Fragments
  • GTP-Binding Proteins

Associated data

  • GENBANK/L24529
  • GENBANK/S53924
  • GENBANK/S54934
  • GENBANK/S54935
  • GENBANK/S54936
  • GENBANK/S54938
  • GENBANK/S54941
  • GENBANK/X73641
  • GENBANK/Z15047
  • GENBANK/Z15048