Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules

Am J Pathol. 1993 Aug;143(2):410-8.


To elucidate the mechanism(s) of myocardial reperfusion injury, we investigated the roles of cell adhesion molecules on both leukocytes and vascular endothelial cells in the reperfused myocardia. We found that within 2 hours after reperfusion leukocytes began to infiltrate into the rat myocardia subjected to 30 minutes of ischemia and clarified, for the first time, that the expression of intercellular adhesion molecule-1 was enhanced on the capillary and venous endothelial cells from 8 to 96 hours after the start of reperfusion. Furthermore, pretreatment with individual monoclonal antibodies against cell adhesion molecules (CD11a, CD11bc, CD18, and intercellular adhesion molecule-1) reduced not only the infiltration of leukocytes but also the area of infarction in the reperfused hearts. These observations suggest that cell adhesion molecules play a critical role in the pathogenesis of myocardial reperfusion injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / administration & dosage
  • Antigens, CD / administration & dosage
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion Molecules / administration & dosage
  • Cell Adhesion Molecules / metabolism*
  • Endothelium, Vascular / metabolism
  • Immunoenzyme Techniques
  • Immunotherapy*
  • Intercellular Adhesion Molecule-1
  • Leukocytes / metabolism
  • Male
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / pathology*
  • Myocardial Infarction / therapy
  • Myocardial Reperfusion Injury / metabolism*
  • Myocardial Reperfusion Injury / pathology
  • Myocardial Reperfusion Injury / therapy*
  • Myocardium / metabolism*
  • Myocardium / pathology
  • Random Allocation
  • Rats
  • Rats, Wistar


  • Antibodies, Monoclonal
  • Antigens, CD
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Intercellular Adhesion Molecule-1