Role of beta 2 integrins and ICAM-1 in lung injury following ischemia-reperfusion of rat hind limbs

Am J Pathol. 1993 Aug;143(2):464-72.


Ischemia/reperfusion involving the hind limbs of rats results in both local injury to skeletal muscle as well as injury to lungs, as measured by increased vascular permeability (125I-labeled bovine serum albumin leakage) and hemorrhage (extravasation of 51Cr-labeled rat erythrocytes). In the current study, we have focused on events in lungs occurring during reperfusion of hind limbs. Analysis of blood neutrophils obtained 4 hours after reperfusion has indicated up-regulation of CD11b and CD18 but not CD11a. Plasma from the same animals demonstrate the ability to induce similar effects in normal blood neutrophils, indicative of the presence of a neutrophil-activating agent in plasma. During reperfusion, lung injury, which develops progressively over a 4-hour period, has been shown to be neutrophil-dependent and requires CD11a/CD18 and CD11b/CD18 as well as intercellular adhesion molecule-1. These data suggest that ischemia and reperfusion injury of rat lower extremities causes systemic changes that result in neutrophil-dependent lung injury that is beta 2 integrin- (leukocyte function antigen-1, Mac-1) and intercellular adhesion molecule-1-dependent.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD / physiology*
  • CD11 Antigens
  • CD18 Antigens
  • Capillary Permeability
  • Cell Adhesion Molecules / physiology*
  • Hemorrhage / metabolism
  • Hemorrhage / pathology
  • Hindlimb / blood supply
  • Integrins
  • Intercellular Adhesion Molecule-1
  • Ischemia / metabolism*
  • Lung / blood supply*
  • Lung / metabolism
  • Lung / pathology
  • Lung Diseases / metabolism
  • Lung Diseases / pathology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Muscles / blood supply
  • Muscles / metabolism
  • Muscles / pathology
  • Rats
  • Reperfusion Injury / metabolism*
  • Up-Regulation


  • Antigens, CD
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Integrins
  • Intercellular Adhesion Molecule-1