Binding of taxol to human plasma, albumin and alpha 1-acid glycoprotein

Res Commun Chem Pathol Pharmacol. 1993 Jun;80(3):337-44.

Abstract

The binding of taxol to human plasma and to individual plasma proteins was studied by equilibrium dialysis. Taxol was found to bind extensively (about 95%) without a significant difference between healthy volunteers and cancer patients. At clinically relevant concentrations (0.1-6 microM), the binding was found to be concentration independent, indicating nonspecific hydrophobic binding. Human serum albumin and alpha 1-acid glycoprotein were found to contribute about equally to the binding, with a minor contribution from lipoproteins. None of the drugs commonly coadministered with taxol (dexamethasone, diphenhydramine, ranitidine, doxorubicin, 5-fluorouracil and cisplatin) altered the binding of taxol significantly. The protein binding of taxol was found to dramatically decrease the red blood cell uptake of taxol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / metabolism*
  • Dialysis
  • Humans
  • In Vitro Techniques
  • Neoplasms / blood
  • Orosomucoid / metabolism*
  • Paclitaxel / metabolism*
  • Plasma / metabolism*

Substances

  • Albumins
  • Orosomucoid
  • Paclitaxel