Epidermal growth factor elevates the activity levels of glycolipid sulfotransferases in renal-cell-carcinoma cells

Int J Cancer. 1993 Sep 30;55(3):448-52. doi: 10.1002/ijc.2910550321.

Abstract

Accumulation of sulfoglycolipids associated with markedly elevated levels of glycolipid-sulfotransferase activity was previously demonstrated in human renal-cell-carcinoma cells. To elucidate the regulatory mechanisms of sulfoglycolipid metabolism in renal-cell carcinoma, effects of various growth factors on the sulfotransferase-activity levels were investigated using a human renal-cell-carcinoma cell line, SMKT-R3. Exogenous epidermal growth factor (EGF) significantly increased the activity levels of the sulfotransferases in a dose-dependent manner, but did not change that of arylsulfatase A, which hydrolyzes sulfoglycolipids. Furthermore, metabolic labeling with 35S-sulfate revealed that the addition of EGF to the culture medium of the cells resulted in an increment of sulfoglycolipid synthesis. The expression of the EGF receptor on SMKT-R3 cells was demonstrated by affinity cross-linking with 125I-EGF. These observations suggest that EGF can regulate sulfotransferase-activity levels in renal-cell-carcinoma cells, and function as one of the regulatory factors of sulfoglycolipid synthesis in these carcinoma cells.

MeSH terms

  • Carcinoma, Renal Cell / chemistry
  • Carcinoma, Renal Cell / enzymology*
  • Cerebroside-Sulfatase / metabolism
  • Epidermal Growth Factor / pharmacology*
  • ErbB Receptors / analysis
  • Glycolipids / metabolism*
  • Humans
  • Kidney Neoplasms / chemistry
  • Kidney Neoplasms / enzymology*
  • Sulfotransferases*
  • Sulfurtransferases / metabolism*
  • Tumor Cells, Cultured

Substances

  • Glycolipids
  • sulfoglycolipids
  • Epidermal Growth Factor
  • ErbB Receptors
  • Sulfurtransferases
  • Sulfotransferases
  • galactosylceramide sulfotransferase
  • Cerebroside-Sulfatase