CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay

Cytometry. 1993;14(6):685-9. doi: 10.1002/cyto.990140614.


We evaluated a flow cytometric method for determining the proportion of CD4-positive T lymphocytes in whole blood using a single three-color tube containing fluorochrome-labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six-tube, two-color assay gated on light scatter parameters. Excellent correlation was found between the three-color analysis using a CD45/side scatter gate and the standard two-color analysis with a light scatter gate. This single-tube three-color test is a promising assay for monitoring CD4+ T-lymphocytes.

MeSH terms

  • Adult
  • Blood Cells / cytology*
  • Blood Cells / immunology
  • CD3 Complex / analysis
  • CD4 Antigens / analysis
  • CD4-Positive T-Lymphocytes / cytology*
  • CD4-Positive T-Lymphocytes / immunology
  • Child
  • Flow Cytometry / methods*
  • Fluorescent Dyes*
  • HIV Seropositivity
  • Humans
  • Leukocyte Common Antigens / analysis
  • Quality Control


  • CD3 Complex
  • CD4 Antigens
  • Fluorescent Dyes
  • Leukocyte Common Antigens