Overexpression of HER2/neu oncogene in human pancreatic carcinoma

Hum Pathol. 1993 Oct;24(10):1127-34. doi: 10.1016/0046-8177(93)90194-l.


The HER2/neu oncogene encodes a transmembrane protein that possesses intrinsic tyrosine kinase activity. Its overexpression has been associated with the malignant phenotype. In this study we examined HER2/neu expression in the normal and cancerous human pancreas. In the normal pancreas HER2/neu immunostaining was observed in acinar and ductal cells. HER2/neu immunoreactivity was expressed in 34 of 76 (45%) pancreatic carcinomas. There was a significant correlation between tumors with well-differentiated histology and HER2/neu expression. Northern blot analysis demonstrated HER2/neu mRNA expression in the normal pancreas and in situ hybridization confirmed its distribution in both acinar and ductal cells. In cancer tissues Northern blot analysis indicated that HER2/neu mRNA levels were elevated in 13 of 25 (52%) of the tumors in comparison with the normal tissues. In addition, in situ hybridization demonstrated a strong but heterogenous distribution of mRNA grains in these tumors. Southern blot analysis did not demonstrate HER2/neu gene amplification in any of the tumors. These data indicate that the HER2/neu protein is synthesized in the normal exocrine pancreas and is frequently overexpressed in well-differentiated adenocarcinomas of the pancreas as a result of increased HER2/neu mRNA levels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Carcinoma / chemistry*
  • Carcinoma / pathology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Oncogene Proteins, Viral / analysis*
  • Pancreas / chemistry*
  • Pancreatic Neoplasms / chemistry*
  • Pancreatic Neoplasms / pathology
  • RNA, Neoplasm / chemistry
  • Receptor, ErbB-2


  • Oncogene Proteins, Viral
  • RNA, Neoplasm
  • Receptor, ErbB-2