A rapid DNA extraction method from culture and clinical samples. Suitable for the detection of human cytomegalovirus by the polymerase chain reaction

Acta Virol. 1993 Feb;37(1):106-8.


We propose an one-step DNA extraction method suitable for the polymerase chain reaction. This procedure utilizes Chelex 100, a chelating in exchange resin. This technique was compared with a traditional technique (proteinase K lysis, phenol-chloroform extraction and ethanol precipitation) for isolation of human cytomegalovirus DNA from clinical samples. The procedure using Chelex 100 appeared to be a simple and fast extraction method for human cytomegalovirus DNA.

Publication types

  • Comparative Study

MeSH terms

  • Bronchoalveolar Lavage Fluid / microbiology
  • Cytomegalovirus Infections / diagnosis*
  • DNA, Viral / isolation & purification*
  • Humans
  • Leukocytes / microbiology
  • Polymerase Chain Reaction*
  • Saliva / microbiology
  • Urine / microbiology


  • DNA, Viral