Contribution of HER-2/neu oncogene expression to tumor grade and DNA content analysis in the prediction of prostatic carcinoma metastasis

Cancer. 1993 Nov 15;72(10):3020-8. doi: 10.1002/1097-0142(19931115)72:10<3020::aid-cncr2820721026>;2-#.


Background: Recent advances in the early detection of prostatic adenocarcinoma have stimulated interest in the development of techniques for determining metastatic potential.

Methods: One hundred cases of adenocarcinoma, including 66 biopsy and radical prostatectomy specimens; 20 biopsies alone; and 14 transurethral resection specimens, were evaluated for Gleason tumor grade, DNA content and HER-2/neu expression. DNA content was determined on Feulgen-stained touch preparations and tissue sections (18 cases) or tissue sections alone. HER-2/neu expression level was determined by image-analysis-assisted quantitative immunocytochemistry.

Results: Tumor grade and ploidy status were independent significant predictors of metastasis. HER-2/neu overexpression was found in 16 (16%) of the 100 cases and significantly correlated with high-tumor grade and aneuploid status, but was not of independent value in the prediction of metastasis.

Conclusions: HER-2/neu overexpression is not uncommon in prostatic adenocarcinoma and is associated with high-tumor grade, abnormal DNA content, and distant metastasis. Tumor grade and DNA ploidy values are of the greatest value in determining the presence of metastasis.

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Aged
  • Aneuploidy
  • DNA, Neoplasm / analysis*
  • ErbB Receptors / genetics*
  • ErbB Receptors / metabolism
  • Gene Expression*
  • Humans
  • Immunohistochemistry
  • Male
  • Neoplasm Metastasis
  • Oncogenes*
  • Predictive Value of Tests
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology*
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism
  • Receptor, ErbB-2


  • DNA, Neoplasm
  • Proto-Oncogene Proteins
  • ErbB Receptors
  • Receptor, ErbB-2