The Polymorphic Epithelial Mucin MUC1 in Human Endometrium Is Regulated With Maximal Expression in the Implantation Phase

J Clin Endocrinol Metab. 1994 Feb;78(2):337-42. doi: 10.1210/jcem.78.2.8106621.

Abstract

After ovulation, progesterone stimulates a temporally regulated secretory transformation in human endometrial epithelium. Using a combination of immunohistochemistry, and Western and Northern blotting, we demonstrate that 1) the polymorphic epithelial mucin MUC1 is secreted by human endometrial epithelium; 2) low levels of both mRNA and core protein are present in the preovulatory phase of the menstrual cycle; 3) mRNA levels increase several-fold after ovulation, consistent with transcriptional regulation by progesterone; 4) there is an increase in translation product in postovulatory endometrium; and 5) the tandem repeat domain of the MUC-1 polypeptide is glycosylated in endometrium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / immunology
  • Blotting, Northern
  • Blotting, Western
  • DNA / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Embryo Implantation*
  • Endometrium / chemistry*
  • Endometrium / metabolism
  • Endometrium / physiology
  • Epithelium / chemistry
  • Epithelium / metabolism
  • Epithelium / physiology
  • Female
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / physiology
  • Glycosylation
  • Humans
  • Immunohistochemistry
  • Membrane Glycoproteins / analysis*
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Menstrual Cycle / physiology
  • Mucin-1
  • Mucins / analysis*
  • Mucins / genetics
  • Mucins / metabolism
  • Neuraminidase / pharmacology
  • Ovulation / physiology
  • Paraffin Embedding
  • Progesterone / pharmacology
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics

Substances

  • Antibodies, Monoclonal
  • Membrane Glycoproteins
  • Mucin-1
  • Mucins
  • RNA, Messenger
  • Progesterone
  • DNA
  • Neuraminidase