Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 242 (3), 263-71

Sequence and Promoter Analysis of the Highly Expressed TEF Gene of the Filamentous Fungus Ashbya Gossypii

Affiliations

Sequence and Promoter Analysis of the Highly Expressed TEF Gene of the Filamentous Fungus Ashbya Gossypii

S Steiner et al. Mol Gen Genet.

Abstract

Ashbya gossypii carries only a single gene (TEF) coding for the abundant translation elongation factor 1 alpha. Cloning and sequencing of this gene and deletion analysis of the promoter region revealed an extremely high degree of similarity with the well studied TEF genes of the yeast Saccharomyces cerevisiae including promoter upstream activation sequence (UAS) elements. The open reading frames in both species are 458 codons long and show 88.6% identity at the DNA level and 93.7% identity at the protein level. A short DNA segment in the promoter, between nucleotides -268 and -213 upstream of the ATG start codon, is essential for high-level expression of the A. gossypii TEF gene. It carries two sequences, GCCCATACAT and ATCCATACAT, with high homology to the UASrpg sequence of S. cerevisiae, which is an essential promoter element in genes coding for highly expressed components of the translational apparatus. UASrpg sequences are binding sites for the S. cerevisiae protein TUF, also called RAP1 or GRF1. In gel retardation with A. gossypii protein extracts we demonstrated specific protein binding to the short TEF promoter segment carrying the UASrpg homologous sequences.

Similar articles

See all similar articles

Cited by 18 PubMed Central articles

See all "Cited by" articles

References

    1. Gene. 1986;45(3):333-8 - PubMed
    1. FEBS Lett. 1990 Jul 16;267(2):245-9 - PubMed
    1. Cell. 1987 Dec 4;51(5):721-32 - PubMed
    1. EMBO J. 1985 Dec 16;4(13A):3539-47 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3648-52 - PubMed

Publication types

Associated data

LinkOut - more resources

Feedback