A brain serine/threonine protein kinase activated by Cdc42 and Rac1

Nature. 1994 Jan 6;367(6458):40-6. doi: 10.1038/367040a0.

Abstract

A new brain serine/threonine protein kinase may be a target for the p21ras-related proteins Cdc42 and Rac1. The kinase sequence is related to that of the yeast protein STE20, implicated in pheromone-response pathways. The kinase complexes specifically with activated (GTP-bound) p21, inhibiting p21 GTPase activity and leading to kinase autophosphorylation and activation. Autophosphorylated kinase has a decreased affinity for Cdc42/Rac, freeing the p21 for further stimulatory activities or downregulation by GTPase-activating proteins. This bimolecular interaction provides a model for studying p21 regulation of mammalian phosphorylation signalling pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / enzymology*
  • Cattle
  • Enzyme Activation
  • GTP Phosphohydrolases / antagonists & inhibitors
  • GTP-Binding Proteins / metabolism*
  • Guanosine Diphosphate / metabolism
  • Guanosine Triphosphate / metabolism
  • Intracellular Signaling Peptides and Proteins
  • MAP Kinase Kinase Kinases
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Binding
  • Protein-Serine-Threonine Kinases / metabolism*
  • Protein-Tyrosine Kinases / metabolism
  • Rats
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • p21-Activated Kinases
  • rac GTP-Binding Proteins
  • rho GTP-Binding Proteins

Substances

  • Intracellular Signaling Peptides and Proteins
  • Saccharomyces cerevisiae Proteins
  • Guanosine Diphosphate
  • Guanosine Triphosphate
  • Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • p21-Activated Kinases
  • MAP Kinase Kinase Kinases
  • STE20 protein, S cerevisiae
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • rac GTP-Binding Proteins
  • rho GTP-Binding Proteins

Associated data

  • GENBANK/U23443