Assignment of human satellite 1 DNA as revealed by fluorescent in situ hybridization with oligonucleotides

Hum Genet. 1994 Feb;93(2):125-8. doi: 10.1007/BF00210595.


We have used a fluorescent in situ hybridization procedure to detect human satellite 1 DNA, the simple sequence family that constitutes the non-male-specific fraction of classical satellite 1 DNA. Satellite 1 appears to be located on pericentromeric regions of chromosomes 3, 4 and 13, and on satellites of each acrocentric chromosome. These results suggest a possible relationship between quinacrine fluorescence of heterochromatin and DNA composition. Furthermore, by means of multicolour in situ hybridization, we have spatially resolved satellite 1 sequences and centromeric alpha-satellite within heterochromatic blocks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Centromere / chemistry
  • Chromosomes, Human, Pair 13 / chemistry*
  • Chromosomes, Human, Pair 3 / chemistry*
  • Chromosomes, Human, Pair 4 / chemistry*
  • Consensus Sequence
  • DNA, Satellite / analysis*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Sequence Data
  • Oligonucleotide Probes*


  • DNA, Satellite
  • Oligonucleotide Probes