Single radial immunodiffusion potency test for standardization of indigenous equine influenza vaccine

A K Gupta; M P Yadav; P K Uppal

Single radial immunodiffusion potency test for standardization of indigenous equine influenza vaccine

Indian J Exp Biol. 1993 Dec;31(12):944-7.

Authors

A K Gupta¹, M P Yadav, P K Uppal

Affiliation

¹ National Research Centre on Equines, Hisar, India.

PMID: 8112772

Abstract

Single radial immunodiffusion (SRD) assays were used for measuring the haemagglutinin antigen contents of equine influenza vaccine prepared from an Indian virus isolate. A/Equine-2/Ludhiana/1/87 (H3N8). Five different preparations of the vaccine were standardized by SRD to prepare 913 doses, each containing 20 micrograms HA/ml-1 dose-1. This test also showed influenza virus subtype specificity as no cross reaction was observed between subtype 1 (H7N7) and subtype 2 (H3N8) viruses.

MeSH terms

Animals
Hemagglutinins, Viral / analysis
Horse Diseases / prevention & control
Horses
Immunodiffusion / methods*
Immunodiffusion / standards
Influenza A virus / immunology*
Influenza Vaccines / analysis*
Influenza Vaccines / standards
Orthomyxoviridae Infections / prevention & control
Orthomyxoviridae Infections / veterinary
Reference Standards
Vaccines, Inactivated / analysis
Vaccines, Inactivated / standards

Substances

Hemagglutinins, Viral
Influenza Vaccines
Vaccines, Inactivated