A monoclonal antibody to calcitonin gene-related peptide abolishes capsaicin-induced gastroprotection

Eur J Pharmacol. 1993 Nov 30;250(1):201-3. doi: 10.1016/0014-2999(93)90645-x.


Calcitonin gene-related peptide (CGRP) released from vasodilator nerves is implicated in the gastroprotective action of capsaicin. This experimental paradigm was used to prove the effectiveness of a monoclonal anti-CGRP antibody. The experiments were performed in anaesthetized rats in which intragastric capsaicin (0.5 mg/kg) reduced gastric injury due to ethanol (50%) by 72%. The protective effect of capsaicin was abolished by close arterial administration of the anti-CGRP antibody #4901 (5 mg) to the stomach. A monoclonal antibody to keyhole limpet haemocyanin was without effect. These data establish anti-CGRP antibody #4901 as a tool to neutralize endogenously released CGRP and show that CGRP is indispensable for the gastroprotective action of capsaicin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / physiology*
  • Calcitonin Gene-Related Peptide / immunology*
  • Calcitonin Gene-Related Peptide / physiology
  • Capsaicin / pharmacology*
  • Ethanol / toxicity
  • Gastric Mucosa / drug effects*
  • Hemocyanins / immunology
  • Injections, Intra-Arterial
  • Male
  • Rats
  • Rats, Wistar


  • Antibodies, Monoclonal
  • Ethanol
  • Hemocyanins
  • keyhole-limpet hemocyanin
  • Calcitonin Gene-Related Peptide
  • Capsaicin