Abstract
mRNAs for novel DNA-binding proteins (GATA-GT1 and GATA-GT2) recognizing the (G/C)PuPu(G/C)NGAT(A/T)PuPy sequence and H+/K(+)-ATPase (proton pump) alpha subunit were detected in parietal cells of the rat gastric body mucosa by in situ hybridization. These results suggest that GATA-GT1 and GATA-GT2 together with H+/K(+)-ATPase are transcribed specifically in gastric parietal cells and that the two DNA-binding proteins may have important roles in cell specific gene regulation. Furthermore, we could detect parietal cells in different states of gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding Sites
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DNA-Binding Proteins / genetics*
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GATA4 Transcription Factor
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GATA6 Transcription Factor
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In Situ Hybridization
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Multigene Family*
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Parietal Cells, Gastric / enzymology
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Parietal Cells, Gastric / metabolism*
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Rats
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Rats, Wistar
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Sodium-Potassium-Exchanging ATPase / genetics*
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Transcription Factors / genetics*
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Transcription, Genetic*
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Zinc Fingers
Substances
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DNA-Binding Proteins
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GATA4 Transcription Factor
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GATA6 Transcription Factor
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Gata4 protein, rat
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Gata6 protein, rat
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Transcription Factors
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Sodium-Potassium-Exchanging ATPase