In this investigation the characteristics of the response to colonic distension of afferent fibres projecting to the inferior mesenteric ganglion (IMG) of the guinea pig were studied in vitro. Intracellular membrane potential recordings were made from neurons in the IMG. Post-synaptic potentials arising from activity in afferent fibres were recorded in these cells following distensions of a small segment of colon placed in a separate and independently perfused organ bath. These afferent fibres showed both transient and sustained responses to distension. Application of a solution containing 0.25 mM Ca2+ and 10 mM2+ Mg2+ to the colon (but not to the IMG) reduced the overall response but did not abolish the activity in these fibres. It is concluded that some afferent fibres which respond to colonic distension project directly to the IMG.