The cloning of CD70 and its identification as the ligand for CD27

J Immunol. 1994 Feb 15;152(4):1756-61.


CD70 is a surface Ag found on activated but not resting T and B lymphocytes. The biologic activity of this Ab-defined cell surface molecule on lymphocytes has not been established. Therefore, in an effort to understand the function of the CD70 protein, a mAb defining the CD70 Ag was used to isolate by expression cloning the cDNA responsible for the CD70 molecule. The predicted protein product is a type II transmembrane protein. Bioassays demonstrated that the CD70 cDNA clone expressed in African green monkey kidney cells would induce the proliferation of PHA-costimulated T cells. Comparison with known sequences indicates identity with the CD27 ligand. Therefore the molecule defining the CD70 Ag is identical to the recently defined ligand for CD27.

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD / biosynthesis
  • Antigens, CD / chemistry*
  • Antigens, CD / genetics
  • Base Sequence
  • CD27 Ligand
  • Cell Line
  • Cloning, Molecular
  • Humans
  • Lymphocyte Activation
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • T-Lymphocytes / immunology
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism


  • Antigens, CD
  • CD27 Ligand
  • CD70 protein, human
  • Membrane Proteins
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Tetradecanoylphorbol Acetate

Associated data

  • GENBANK/S69339